مع اطيب امنيا تي لكم بالتوفيق

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1 Philadelphia University Faculty of Science Department of Biotechnology & Genetic Engineering Lecturer: Dr. Marwan Abu-halaweh Student Name: Student Number: Course Name: Animal Biotechnology Course Number: First Semester 2009/2010 Date: 08/04/10 Time: 9:10 First Exam Duration: 50 minutes Number of Pages: 5 Number of Questions: 4 Mark: /20 / #### مع اطيب امنيا تي لكم بالتوفيق 1

2 I- Chose the correct answer: (2 points each) 1-The development of tissue culture as a useful tool in the modern laboratory arose largely because of the needs of the following: A- The production of antiviral vaccine. B- Cancer research C- A and B are corrects D- None of the above is corrects. 2-Which of the following is/are not considered as advantage of tissue culture: A- The remains homogeneity of cell types (Achieved through serial passage). B- It is economical because smaller quantities of reagents are needed compare to in vitro. C- Unstable aneuploid chromosome constitution. D- A and B are corrects E- A and C are corrects. 3- Which of the following is/are considered as disadvantage of tissue culture A. It needs controlled physiochemical environment (ph, Temperature, osmotic pressure. O2, CO2, etc. B. The legal, moral and ethical questions of animal experimentation can be avoided. C. Unstable aneuploid chromosome constitution. D. A and B are corrects E. A and C are corrects 4- Which of the following is/are considered as a role/ function of genes? A- To encode the nucleotide sequences of mrna. B- To encode the nucleotide sequences of trna. C- To regulate the expression of other genes. D- All of the above are corrects. E- None of the above are corrects. 5- Which of the following is not correct for restriction enzymes type I A- After traveling bp the enzyme cleaves only one strand of the DNA randomly B- Those enzymes create a gap of about 75 bp in length. C- This kind of Enzyme is useful for gene manipulation because their cleavage sites are not specific. D- A and B are corrects E- None of the above is corrects 2

3 6- Which of the following is correct/s for restriction enzymes type II? A. Enzyme belong to this group are used in gene manipulation. B. Those enzymes recognize a particular target sequence in a double stranded DNA molecule. C. Those enzymes do not require Mg+2 ions for restriction (cutting). D. A and B are corrects. E. B and C are corrects. 7- Yeast artificial chromosome are A- Self-replicating elements B- Able to insert segments up to 400 kb. C- Can replicate any inserted DNA via transfer to yeast cells. D- D- A, B and C E- None of the above 8- Which of the following is/are a function/s of TFIIH in transcription process: A- Act as helicase which unwinds the DNA. B- Also act as kinase that adds phosphate groups to the C-terminal domain of the largest subunit of RNA polymerase II. C- recognizes the TATA components of the RNA polymerase II core promoter. D- A and B E- A, B and C 9- Which of the following is correct for Poly A tail. A- Virtually all eukaryotes have a series of up to 250 bp of adenosines at the 3` ends. B- These are not part of the template DNA. C- These sequence are added to the transcript by a template independent RNA polymerase called poly(a) polymerase. D- A and B E- A, B and C 10- Cloning is a form of reproduction. A- Sexual B- Asexual C- A and B D- Neither A nor B II- Fill the brackets with true for the correct sentences and false for the incorrect ones. (2 points each) ( ) 1- It was found that serum should be supplemented with 50-70% to provide completely adequate medium for the continuous propagation of established cell lines. ( ) 2- DNA ligase allows joining together any two DNA fragments. ( ) 3-Phage vector can insert up to 35 kb DNA fragments ( ) 4- Nucleotide which is used in sequencing reaction is known as deoxynucleotide ( ) 5- mrna strand can form a double-stranded hybrid with a DNA strand. ( ) 6- The ideal buffer should interfere with chemical or biochemical process of the culture. III- Fill in the blank with the correct answer. (2 points each) 1- What is Animal Biotechnology? 3

4 2- A nucleotide consists of, and. 4-- Capping of RNA polymerase is an important step in where an extra is added to the extreme 5`-end of the RNA. 5- Large Ribosomal subunit is also called 60S subunit composed of, and. V- Short answers questions. (Chose one question from A and B to answer) A- Summarized RNA translation elongation process? (12 points) B- As a biotechnologist your supervisor asks you to establish a cell line from liver tissue in point format explains the protocol you used. (12 points) C- Explain how eukaryote cell control its RNA processing? (6 points) 4

5 D- Open tissue culture vessel are kept in humidified incubator is this statement correct or not? Explain why? (6 points) E-The basic principle of particle bombardment for gene transfer the most gene transfer technique used in animal and human is this statement correct or not explain why?(6 points) F-Cosmid is a natural vector is this statement correct or not? Explain how you can construct such vector? (6 points) 5